Effect of atrial natriuretic peptide ( ANP) on pulmonary melanoma metastasis in ANP knockout mice and C57BL／6J mice / 中国比较医学杂志

Objective To investigate the role of atrial natriuretic peptide (ANP) in lung metastasis of melanoma. Methods Melanoma B16F10 cells were intravenously injected into ANP knockout mice and C57BL／6J mice. After three weeks, the mice were sacrificed, and the lungs were removed, embedded, and examined by pathology using HE staining. The numbers of metastatic foci on the lung surface and micrometastatic foci in the lung tissues were counted. Results The ANP knockout mice displayed fewer metastatic foci on the lung surface and less micrometastatic foci in the lung tissues of ANP knockout mice than in the C57BL／6J mice. Conclusions ANP deletion significantly suppresses the metastasis of melanoma in the lung of mice.

Inhibitory effect of atrial natriuretic peptide(ANP)on the tumor growth of ANP knockout mouse and C57BL/6J mouse models of subcutaneous xenotransplanted melanoma / 中国比较医学杂志

Objective To study the effect of atrial natriuretic peptide(ANP)deletion on melanoma growth. Methods A subcutaneous xenotransplanted melanoma model was established in ANP knockout mice and C57BL/6J mice. The tumor volume was measured at the sixth day after establishment of the subcutaneous transplantation. Tumor cell proliferation and tumor-induced angiogenesis were detected by immunohistochemical staining. Results The volume and weight of xenotransplanted melanoma were significantly decreased in the ANP knockout mice compared with those in the C57BL/6J mice. The proliferative tumor cells and microvessel density were significantly decreased in the tumor tissues of ANP knockout mice compared with those in the C57BL/6J mice. Conclusions ANP deletion significantly suppresses xenotransplanted melanoma growth through inhibiting the tumor cell proliferation and angiogenesis.

Treatment of distal tibia fractures with intramedullary nail or plate: a meta-analysis

To evaluate the outcome of intramedullary nail and plate fixation for the treatment of extra-articular fractures of the distal tibia and to determine whether there are sufficient objective data in the literature to compare the two methods. A comprehensive search of all relevant articles from Jan 1975 to Dec 2011 was conducted. Two reviewers evaluated each study to determine its suitability for inclusion and collected the data of interest. Meta-analytic pooling of group results across studies was performed for the two treatment methods. The systematic review identified 22 primary studies with 880 fractures including 15 groups of intramedullary nail and 15 groups of plate. For extra-articular distal tibia fractures, shorter healing time can be achieved by using the intramedullary nail, but the malformation rate was significantly higher than in the plate group. The average operating time in the intramedullary nail group was longer than in the plate group, but the difference was not statistically significant. No statistically significant difference was found when comparing the rates of infection, rotation, shortening, delayed union and nonunion. The reoperation rate was higher in the intramedullary nail group compared with the plate group, but the difference was also not statistically significant. The functional and efficacy outcomes appear to be similar between the two treatment groups. Thus the patient's general condition and the surgeon's preference dictate the choice of surgical technique